POKEWEED MITOGEN-, CONCANAVALIN A-, AND PHYTOHEMAGGLUTININ-INDUCED DEVELOPMENT OF CYTOTOXIC EFFECTOR LYMPHOCYTES
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چکیده
منابع مشابه
Pokeweed Mitogen-, Concanavalin A-, and Phytohemagglutinin-induced Development of Cytotoxic Effector Lymphocytes
Cultures of chicken lymphoid tissues were tested for their capacity to lyse (51)Cr-labeled chicken, burro (BRC), and human red blood cells (HRC) in the presence of phytomitogens. PHA-stimulated cultures lysed all three types of targets, while PWM and Con A showed a "target cell specificity" for HRC and BRC, respectively. In mixtures of target cells only the appropriate targets were lysed by lym...
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Blast transformation of lymphocytes from diabetics and nondiabetics was evaluated after adding insulin at various concentrations. Responses to phytohemagglutinin-P (PHA-P), concanavalin-A (CON-A) and pokeweed mitogen (PWM) were measured in the presence of exogenous insulin added in physiologic increments of 0, 10, 20, 30, and 40 microunits of activity per ml of culture medium. A modified method...
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Activation of T lymphocytes by pokeweed mitogen (PWM) is strictly monocyte (Mo)-dependent and results in T-cell mitogenesis and interleukin-2 (IL-2) secretion, coupled with an inability to utilize IL-2 due to an impaired expression of functional IL-2 receptor (IL-2R). Such IL-2R impairment could arise in PWM-activated T cells themselves or, alternatively, be the result of Mo-derived influences,...
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The present study demonstrates that human peripheral blood mononuclear cells (PMC) can be stimulated in vitro to become cytotoxic T lymphocytes (CTL) by PHA. A significant cytotoxic activity of PMC was detected 48 hr after the culture initiation in the presence of 5 micrograms/ml of PHA and the peak level of the activity was obtained by culturing PMC for 72 hr. The cytotoxic cells require the p...
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Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transformation stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorporation in to target cells after interaction with PHA-treated PBL. The cytotoxicity of P...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1973
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.138.4.812